The prevalence of KRAS and BRAF mutation in colorectal cancer patients in Bali
نویسندگان
چکیده
Mutations in the KRAS (Kirsten rat sarcoma viral oncogene homolog gene) and BRAF (v‐Raf murine B1) gene play a significant role primary resistance to colorectal cancer therapy. Around 85‐90% of mutations occur exon 2 (codon 12 13), whereas approximately 96% 15 codon 600 (V600E). This study aimed determine prevalence mutation characteristics genes patients Bali. The DNA was isolated from 44 formalin‐fixed paraffin‐embedded samples which were stored Department Pathology, Sanglah General Hospital 2017. Detection carried out by polymerase chain reaction (PCR) direct sequencing. Out samples, only 27 successfully amplified sequenced. Our findings showed six (22.2%) with mutated at codons 13 (including two G12D, one sample G12V, three G13D). Interestingly, we found (11.1%) mutation, including V600E V600L mutation. Taken together, our results that identified occurred exclusively. Further studies are essential identify correlation these prognosis response chemotherapy
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ژورنال
عنوان ژورنال: Indonesian Journal of Biotechnology
سال: 2022
ISSN: ['0853-8654', '2089-2241']
DOI: https://doi.org/10.22146/ijbiotech.67506